Microbiota DNA was. Molecular Devices Spectramax M5 Microplate Reader. SpectraMax® M4. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Figure 4: Transcreener ADP. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. 96-1536. Then, the fluorescence change rates (F 0 − F)/F 0 were calculated, and the selectivity histogram. Microplate Reader Documentation Version No. Catalog Number: (89212-396) Supplier: Molecular Devices. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. The cells were then treated to induce apoptosis. Molecular Devices SpectraMax® series microplate readers. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Designed to provide the simplicity, flexibility and power required for advanced data analysis. Go to Product Details. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. B: Selection of Excitation Wavelength. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. 6: bioNova científica, s. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The PBS solution (10. 67. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. All associated StakMax® stackers. 6 for further experiment, so I'm confused which i would choose for OD value determination. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. com A. ® reader to the computer. Ltd. anisotropy was measured on SpectraMax M5 microplate reader with 485 nm excitation and 535 nm emission after 60 min incubation at room temperature. Recommended Optics Wavelength (nm) Wavelength. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The assay window spanned four orders of magnitude. Sensitive fluorescent quantitation of DNA with the Quant-iT. 0 μL) instead of the sample solution was applied as control group, and. The raw data from each individual experiment were normalized to vehicle-treated HK-2. 58% for the detection module and 2. 0 OD for earlier models. Download PDF. As. 73. 1. In brief, RAW 264. To quantify the A1AT in rat serum, standard curves were drawn for nA1AT, rA1AT-WT and rA1AT mutants, respectively. Molecular Devices Spectramax M5 Microplate Reader. Measurements of fluorescence and fluorescence polarization were performed on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA) with the excitation and emission wavelengths 544 nm and 590 nm, respectively. 67. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Microplate Readers are modular and . The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. Plates were then shaken at room temperature for 10 min and the absorbance at 570/630 nm was measured by using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). SpectraMax® ABS. M3-M5e_manual. Spectramax M5 Microplate Reader, supplied by Danaher Inc, used in various techniques. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. The cuvette must be in place when you read the plate. 2. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. view more . metrically at 540 nm using a microplate reader (SpectraMax 384 Plus, Molecular Devices Corp. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Other technologies such as imaging. Qualify the luminescence performance of the SpectraMax . Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Plate Shaking. 14 Average %CV 0. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. Five. Learn how you can utilize microplate readers to drive landmark discoveries. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe reader’s stated range. Page 28 Chapter 5: Read Modes and Read Types 1. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. book Page 7 Wednesday, July 7, 2010 2:09 PM. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 5–100mg/L (ValitaTiter) or 100–2000mg/L (ValitaTiter Plus). 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit microplate reader. Microplate reader settings. Self Service Available. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. 313 reference gain check fail. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Molecular Devices SpectraMax™ Plus 384 Microplate Reader (s) with One-Year Warranty $9,000. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. 6-384 (96- and 384-well with pipetting) 6-384. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. The absorbance values at 450 and 570 nm were determined using the SpectraMax M5 microplate reader (Molecular Devices, Silicon Valley, CA, United States). SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Bookings are free, but you will need to use iLab to book. 04 to 3. Add to Cart. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. When a. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. The chamber contains springs that position the cuvette in the proper alignment for a read. 0 out of. Instrument settings are shown in Figure 3. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. RAW 264. Fluorescence Microplate Readers. 312 gain calibration failed. SpectraMax Microplate Readers. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Download PDF. Bookings are free, but you will need to use iLab to book. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. It cannot be used for luminescence measurements. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. One vial of Thaw-and-Use TIGIT Effector Cells was thawed and resuspended in cell recovery. TNF standard curve. #M5) SpectraMax® L Microplate Reader (cat. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. 2 only) SpectraMax M-series Microplate readers. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. 40266 Email: drugdiscoverytech@lifetech. Step 1. 49 2. Three independent experiments were performed. 315 can't find zero order. The acceptance. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 3 1. The spectrophotometric. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. Data are normalized to vehicle-treated. 95. System Diagrams and Dimensions. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. These lab workhorses are used in drug discovery, research, bioassay validation, and. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. We would like to show you a description here but the site won’t allow us. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Molecular Devices. Detailed specifications of each model reader are reported in Section 7, “Specifications”. "With this complete portfolio of multi-mode configurations, we. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . We would like to show you a description here but the site won’t allow us. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. When a feature or capability applies to only certain readers, this exception is noted. The plate reader can measure multiple readouts, including UV and visible absorbance. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. Compatible automation solutions for SpectraMax Readers. Shop now. Maximum signal with lowest background. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The cuvette must be in place when you read the plate. Figure 1. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. The SpectraMax M5e Multi. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 2. Temperature variation ± 0. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 6% for the on-board optics (monochromators). 1 & 7. Microplate Readers. 305 reference level saturation. M3-M5e_manual. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. 0 . Bioz Stars score: 86/100, based on 1 PubMed citations. View the Molecular Devices website. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. Materials. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Bookings are free, but you will need to use iLab to book. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. com 13. The CDWIA was performed on each. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Spectramax Multi-Mode Microplate Reader Performance Specifications. Please refer to the Obsolete Microplate Reader Models for more information. analysis, controls the SpectraMax Multi-Mode Microplate Readers. The drawing shows a standard microplate of 15 mm height covered with a lid. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. The SpectraMax M2 and M2e are versatile and reliable. Ferguson, Ph. SpectraMax® M5 Multi-Mode Microplate Reader (cat. Table of Contents. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Page 28 Chapter 5: Read Modes and Read Types 1. 2. The assay applies fluorescence polarization to quantify Fc-containing IgG. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 2. Do not touch the clear surfaces of the cuvette. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. However, when a specific probe is not available, or it causes off-target effects on the. You should get comparable results from top and bottom reads using EC50's to compare reads. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. 5 12. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. The concentration of antibodies was determined by using a spectrophotometer from Puxi General Instrument Co. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The SpectraMax M2 and M2e are versatile and reliable. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. es •. Choose from a. 72 MB. CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). both cuvette-based and microplate reader applications on the same instrument. The guide also covers the software features, the specifications, and the accessories of the systems. The SpectraMax® ABS Plus Microplate Reader can run . The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. For the Live/Dead assay, they were treated. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] or greater. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. Italic and boldface type are used for emphasis. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. . Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 72 MB. Fluorescence Microplate Reader Comparison Chart. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The following document is intended to demonstrate setup of this instrument. 2. View the Molecular Devices website. Microplate Reader Shelf. l. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Microplate Readers include the M3, M4,. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. AquaMax® 4000. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. reader in the visible range between 405–750 nm with NIST and NMI. 43 Max %CV 1. Five preferred modes for diverse bioresearch applications. FlexStation™ II. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax M5 reader settings are shown. 1 Version 7. Western blotting assays were conducted according to previous studies . , USA). Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. Detailed specifications of each model reader are reported in Section 7, “Specifications”. SpectraMax M5 Multi-Mode Microplate Reader. A control experiment of the activity of the proposed enzyme/ZIF-8A particles without peptides against protease digestion was also performed. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. 2. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 6: bioNova científica, s. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The drawing shows a standard microplate of 15 mm height covered with a lid. Add to Cart. Compact and tunable absorbance microplate readers DATA SHEET. SpectraMax® Aquamax. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Learn how to set up protocols, customize reports, export results, and more with this PDF document. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Wide range of applications. Thank you!. Microplate Readers include the M3, M4, M5, and M5e models. 6 for further experiment, so I'm confused which i would choose for OD value determination. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. Background fluorescence has been subtracted. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Microplate Readers are modular and . The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. S. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Figure 1. Between steps, reactions were rinsed 3 times with TBS + 0. used. Use similar settings for other Molecular Devices readers with fluorescence detection mode. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 304 signal level saturation. When a FlexStation® 3 Multi-Mode Microplate Reader. No additional manuals are available. The system. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Access this by going to Help >. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. AlphaScreen. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. The dual monochromator optics allow the widest range of applications to be. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). Microplate Readers include the M3, M4, M5, and M5e models. Expanded wavelength coverage. Depending on which. B: Selection of Excitation Wavelength. 9000-0756. FlexStation™ I. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Page 1 of 8 . 00 USD. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. . SoftMax Pro Software has been integrated by many leading robotics andFor readers without a cuvette port, simply check the box next to ‘PathCheck’. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. SpectraMax® M5. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. 7°C and the bottom to 36. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 303 unable to cal dark current. VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® Plus 384 Absorbance Microplate Reader; SpectraMax® L (software version 5. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Table of Contents. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Fill a clean plate with water. Qualify the fluorescence performance of the SpectraMax . IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening.